• ion channel Another surprising observation described in this

  2018-10-22

  

  Another surprising observation described in this report is that, rather than being constitutively expressed with respect to the ion channel during differentiation, developmental regulators retained a strong expression signature linked to transition through G1. This again supports the idea that G1 r

• Recent studies indicated that the

  2018-10-22

  

  Recent studies indicated that the number of grafted dopamine neurons required to produce functional recovery in 6-OHDA-lesioned rats is relatively low (Grealish et al., 2015). In the present study, we report an average of ∼21% cell survival from cryopreserved cells. This fraction is similar to what

• Among the potential stem cell candidates

  2018-10-20

  

  Among the potential stem cell candidates for therapeutic development, we have highlighted the existence of a promising multipotent contender residing within the nasal olfactory mucosa (Delorme et al., 2010; Murrell et al., 2005; Tome et al., 2009), a peripheral and permanently self-renewing nervous

• br Materials and methods br Acknowledgment This work

  2018-10-20

  

  Materials and methods Acknowledgment This work was supported by the Foundation for Polish Science: Welcome program [grant no.: 2010-3/3 to Maciej Wiznerowicz] and the National Science Centre [grant number 2012/07/E/NZ3/01819]. The authors wish to thank Prof. Gustavo Mostoslavsky from Boston Un

• In vivo the stiffness and

  2018-10-20

  

  In vivo, the stiffness and density of the surrounding ECM present an additional physical challenge to the moving cell body. Two principal mechanisms are known for moving cells to overcome these constraints: (i) proteolytic ECM degradation leading to gap widening and cell-generated trail formation an

• br Materials and methods br Results and discussion br Acknow

  2018-10-20

  

  Materials and methods Results and discussion Acknowledgments Introduction Reprogramming rejuvenates aged somatic cells back into the pluripotent state (Takahashi et al., 2007; Takahashi and Yamanaka, 2006). The developmental plasticity of induced pluripotent stem cells (iPSC) demonstrate

• gaba b Recent methods of reprogramming human fibroblasts to

  2018-10-20

  

  Recent methods of reprogramming human fibroblasts to iNSCs employed genome-integrating lentiviral or retroviral vectors (Kumar et al., 2012; Ring et al., 2012), which may produce insertional mutations and residual or reactivation of transgenes. We used oriP/EBNA-1 episomal vectors to deliver reprogr

• In contrast to bona fide ESC lines

  2018-10-20

  

  In DIG-11-dUTP to bona fide ESC lines, the pre-ESC lines expressed H19 and IGF2 mRNAs and lower levels of DNMT3B. This may reflect their limited ability to differentiate into the three germ layers, as a high H19 expression level has been associated with altered differentiation potential in parthenog

• br Materials and methods br Acknowledgements br

  2018-10-20

  

  Materials and methods Acknowledgements Resource table Resource details To generate the Saudi KACST induced Pluripotent Stem Cell line No 1 (SKiPSc1) cell line, four reprogramming Yamanaka factors (Oct3/4, Sox2, c-Myc, and Klf4) (Takahashi et al., 2007; Yu et al., 2007) were delivered to

• br Author disclosure statement br Acknowledgements Authors w

  2018-10-20

  

  Author disclosure statement Acknowledgements Authors would like to thank Dr. T. Ramakrishna Murti for his valuable comments and linguistic corrections in the manuscript. Authors would also like to thank D.I Biotech (Seoul, Korea) for support to analyze neurite outgrowth. This work was supporte

• In contrast to the findings that every

  2018-10-20

  

  In contrast to the findings that every iPSC line examined in this and previous studies harbored protein-coding mutations, we did not detect any protein-coding mutation in one SCNT-ESC line (Table 1, SCNT2). Moreover, there were two pairs of SCNT-ESC lines (SCNT3 and SCNT4 and SCNT6 and SCNT10) that

• br Funding This work was supported

  2018-10-20

  

  Funding This work was supported by Department of Neurosurgery, the University of Texas Health Science Center at Houston, Memorial Hermann Foundation-Staman Ogilvie Fund, the Bentsen Stroke Center, the TIRR Foundation through Mission Connect (No. 014-115), Craig H. Neilsen Foundation (No. 338617),

• Our studies show that only a subpopulation of hESCs

  2018-10-20

  

  Our studies show that only a subpopulation of hESCs at the top of the hierarchy possesses an extensive capacity for self-renewal. Polanco et al. recently showed that potassium channel in this compartment could reestablish the continuum of cell states seen in unfractionated cultures (Polanco et al.,

• We exploited the fact that the eHBs

  2018-10-20

  

  We exploited the fact that the eHBs were trapped as progenitors to analyze the effect of specific growth factors upon them and uncovered a central role for FGF (Figure 6). These data are consistent with studies in which FGF promoted the expansion of BL-CFCs derived from human or murine ESCs (Faloon

• br Experimental Procedures br Acknowledgments We

  2018-10-20

  

  Experimental Procedures Acknowledgments We thank Ms. Y. Ogata for technical assistance. Financial support for this research was provided by The Uehara Memorial Foundation, and The Ministry of Education, Culture, Sports, Science, and Technology of Japan and the Japan Science and Technology Agen

15712 records 1047/1048 page Previous Next First page 上5页 104610471048